基于高通量转录组测序的山羊睾丸和附睾头差异表达基因分析

doi:10.11843/j.issn.0366-6964.2014.03.008

Abstract

Abstract:

The objective of this study was to find the differentially expressed genes between testis and epididymis caput in adult bucks，which was necessary to better understand mechanisms involved in spermatogenesis and sperm maturation.Testis and epididymis caput of three adult bucks known fertility were isolated and their mRNA were respectively extracted.The transcriptomes were analyzed by Illumina HiSeq^TM 2000 high-throughput RNA sequencing.All of Unigenes were annotated using the BLAST search against Nr，GO and KEGG databases.The differentially expressed genes were enriched in gene ontology and KEGG pathway.The results showed that 57 727 and 51 395 Unigenes were respectively in caprine testis and epididymis caput; There were 25 201 Unigenes annotated into database of molecular function，25 456 Unigenes into cellular component，27 318 Unigenes into biological process，of which there were 9 150，9 172 and 10 003 the differentially expressed genes respectively; There were 23 222 Unigenes annotated into KEGG pathways，including 8 219 the differentially expressed genes.The signal pathways that differentially expressed genes clustered significantly were mRNA surveillance pathway and mTOR signaling pathway; There were 24 592 the differentially expressed genes in caprine testis and epididymis caput，in which 8 420 genes were up-regulated and 16 172 genes were down-regulated.The top three genes of all up-regulated genes in epididymis caput were beta-defensin 124、beta-defensin 109 and beta-defensin 108，whereas the top three genes of all down-regulated genes were gametocyte specific factor 1-like，Serine/arginine repetitive matrix 1 and fibronectin type III domain containing 8.The three of the highest expressed genes in epididymis caput were lipocalin 5，beta-defensin 124 and glutathione peroxidase 5.The transcriptomes in testis were more than those in epididymis caput; The family of bete-defensins，lipocalins and GPx5 may play important roles in sperm maturation，storage and fertilization.

CLC Number:

S827.2

ZHANG Chun-xiang，ZHANG Guo-lin，GUO Li-na，ZHAO Hui，REN You-she. Study on Differentially Expressed Genes between Caprine Testis and Epididymis Caput Based on Transcriptomes with High-throughput RNA-seq Technology[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 391-401.

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Study on Differentially Expressed Genes between Caprine Testis and Epididymis Caput Based on Transcriptomes with High-throughput RNA-seq Technology

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